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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Tacuarembó. |
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Fecha actual : |
19/09/2014 |
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Actualizado : |
15/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
DALLA RIZZA, M.; REAL, D.; REYNO, R.; QUESENBERRY, K; BURGUEÑO, J; PORRO, V; ERRICO, E. |
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Afiliación : |
MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; DANIEL REAL FERREIRO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Genetic diversity and DNA content of three South American and three Eurasiatic Trifolium species |
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Fecha de publicación : |
2007 |
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Fuente / Imprenta : |
Genetics and Molecular Biology, 2007, v 30, no. 4, p.1118-1124. |
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DOI : |
10.1590/S1415-47572007000600015 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: March 16, 2007 / Accepted: May 17, 2007.. |
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Contenido : |
Six species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T. polymorphum). Genome size values of South American species were higher than those of Eurasiatic origin. The analyses of the molecular data grouped the six species in agreement with their geographical origin and clearly differentiate T. polymorphum from T. argentinense. The Eurasiatic group showed the highest average of species-specific bands (45.3%) and the South American group exhibited the highest amount of total bands (59.7). The highest level of intra-species polymorphisms was detected in T. argentinense (92.9%), followed by T. medium(89.5%). |
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Thesagro : |
ADN; PASTURAS; TRIFOLIUM; VARIABILIDAD GENETICA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02106naa a2200265 a 4500
001 1052593
005 2019-10-15
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1590/S1415-47572007000600015$2DOI
100 1 $aDALLA RIZZA, M.
245 $aGenetic diversity and DNA content of three South American and three Eurasiatic Trifolium species
260 $c2007
500 $aArticle history: Received: March 16, 2007 / Accepted: May 17, 2007..
520 $aSix species of Trifolium (T. polymorphum Poir., T. riograndense Burkart, T. argentinense Speg., T. medium L., T. pratense L. and T. repens L.) were analyzed using inter-simple sequence repeats (ISSR) markers. Six selected primers generated 186 polymerase chain reaction (PCR) products exploring 112 loci in 34 genotypes analyzed with molecular sizes ranging from 200 to 1300 bp. These primers were able to discriminate among and within species, with the PCR products being on average 41.6% species-specific and 59.9% polymorphic at the within species level. Nuclear DNA content was determined by flow cytometry and revealed variation among species. The 1Cx genome size values were calculated and were found to range from 0.46 pg (T. pratense) to 0.96 pg (T. polymorphum). Genome size values of South American species were higher than those of Eurasiatic origin. The analyses of the molecular data grouped the six species in agreement with their geographical origin and clearly differentiate T. polymorphum from T. argentinense. The Eurasiatic group showed the highest average of species-specific bands (45.3%) and the South American group exhibited the highest amount of total bands (59.7). The highest level of intra-species polymorphisms was detected in T. argentinense (92.9%), followed by T. medium(89.5%).
650 $aADN
650 $aPASTURAS
650 $aTRIFOLIUM
650 $aVARIABILIDAD GENETICA
700 1 $aREAL, D.
700 1 $aREYNO, R.
700 1 $aQUESENBERRY, K
700 1 $aBURGUEÑO, J
700 1 $aPORRO, V
700 1 $aERRICO, E.
773 $tGenetics and Molecular Biology, 2007, v 30, no. 4, p.1118-1124.
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